OPTICAL FRACTIONATORĮstimate individual cell volum es with the nucleator. Note: avoid counting pieces of cells when you really want to count whole cells. Disector counting rules are followed to avoid overestimating, and an oil objective lens is employed for imaging, since fine z-resolution is needed to find the leading edge of the cell and to have enough focal planes to determine if it is in the disector. A virtual space called an optical disector is used in thick sections that can be oriented anyway you like. OPTICAL FRACTIONATOR to estimate NUMBER of cellsĮstimate the size of cell populations with the optical fractionator in thick tissue sections. In this probe, sub-volumes are sampled and then are extrapolated to arrive at an estimate of the entire cell population. These probes should always be used in conjunction with systematic random sampling. These stereological probes are appropriate for many fields of basic and applied biological and medical research. The most modern unbiased stereology probes are used to quantify aspects of biological tissue in a reproducible and efficient manner. Unbiased stereology provides an important contribution to the advancement in biological research by improving the consistency and dependability of quantitative analytical results produced in the laboratory and reported in scientific publications. It produces results that are unbiased, efficient, and more reliable than other ad hoc quantitative analyses. When properly used, stereology plays an important role in validating and rejecting experimental hypotheses in biological research. What is Stereology?ĭesign-based stereology is a set of methods to ensure rigorous quantitative analysis of the size, shape, and number of objects.
This site is dedicated to helping researchers understand the principles of design-based stereology and its advantages over less sophisticated approaches in quantitative histology. The application of stereological methods to biological studies permits researchers to effectively and efficiently gather unbiased, accurate data. The emphasis is on the use of stereology in biological research, though anyone interested in learning concepts of stereology will find something of interest.
This site introduces both basic and advanced concepts of Stereology. Email Stereology Information for the Biological Sciences
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